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A Petri dish named after the German bacteriologist Julius Richard Petri, is a shallow cylindrical glass or plastic lidded dish that biologists use to culture cells.

Modern Petri dishes usually feature rings and/or slots on their lids and bases so that when stacked, they are less prone to sliding off one another. Multiple dishes can also be incorporated into one plastic container to create a "multi-well plate". While glass Petri dishes may be reused after sterilization (via an autoclave or one hour's dry-heating in a hot-air oven at 160 °C, for example), plastic Petri dishes are often disposed of after experiments where cultures might contaminate each other.

Petri dishes are often used to make agar plates for microbiology studies. The dish is partially filled with warm liquid containing agar and a mixture of specific ingredients that may include nutrients, blood, salts, carbohydrates, dyes, indicators, amino acids or antibiotics.

Once the agar cools and solidifies, the dish is ready to be inoculated ("plated") with a microbe-laden sample. Virus or phage cultures require a two-stage inoculation: after the agar preparation, bacteria are grown in the dish to provide hosts for the viral inoculum.